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Scatter plot of spot intensity from the cDNA array on log2 scale where the signal from the Cy5 channel (tester) was plotted against the <t>Cy3</t> channel (driver). Data from the images of both Cy3 and Cy5 were plotted as the mean signal intensity after normalization of the transcripts spotted in six replicates. The data points cluster around a ratio of 0, representing equal level of expression. The solid line (middle) marks a 1-fold increase (+) or decrease (−) in the Cy5/Cy3 ratio. The dashed lines indicate the 2-fold induction (or repression) cutoff level.
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Scatter plot of spot intensity from the cDNA array on log2 scale where the signal from the Cy5 channel (tester) was plotted against the Cy3 channel (driver). Data from the images of both Cy3 and Cy5 were plotted as the mean signal intensity after normalization of the transcripts spotted in six replicates. The data points cluster around a ratio of 0, representing equal level of expression. The solid line (middle) marks a 1-fold increase (+) or decrease (−) in the Cy5/Cy3 ratio. The dashed lines indicate the 2-fold induction (or repression) cutoff level.

Journal: Frontiers in Genetics

Article Title: Analysis of plant expression profiles revealed that aphid attack triggered dynamic defense responses in sorghum plant

doi: 10.3389/fgene.2023.1194273

Figure Lengend Snippet: Scatter plot of spot intensity from the cDNA array on log2 scale where the signal from the Cy5 channel (tester) was plotted against the Cy3 channel (driver). Data from the images of both Cy3 and Cy5 were plotted as the mean signal intensity after normalization of the transcripts spotted in six replicates. The data points cluster around a ratio of 0, representing equal level of expression. The solid line (middle) marks a 1-fold increase (+) or decrease (−) in the Cy5/Cy3 ratio. The dashed lines indicate the 2-fold induction (or repression) cutoff level.

Article Snippet: The microarray slides were scanned in both 635 nm (Cy5) and 532 nm (Cy3) channels using a ScanArray Express Scanner (Perkin Elmer Applied Biosystems, Foster City, CA), and spot detection and quantification were carried out using GenePix Pro 4.0 software.

Techniques: Expressing